Lentiviral vector production using single-use bioreactors
A robust and scalable process in HEK293T cells with HyClone™ peak expression medium for growth and transfection.
Primary clarification of lentiviral vectors
In this study, we discuss development of a primary clarification protocol to separate LV from bioreactor-derived suspension cells and debris using a scalable filter train.
Improving lentiviral vector downstream processing workflows
In this article we discuss process development pain points of a modern downstream workflow and ways to address them.
Lentivirus purification
In this study, we share an alternative lentivirus purification process using capture with weak anion exchange and optimization of the Capto™ Core 700 resin polishing step to maximize infectious recovery.
ÄKTA ready™ 450
Single-use chromatography system for small-scale manufacturing.
Capto™ Core 700 resin
Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality.
Stax™ disposable depth filter
Highly versatile disposable depth filter platform.
Xcellerex™ XDR bioreactor
Single-use bioreactor systems providing scalable and robust stirred-tank performance for up to 2000 L in both cGMP and non-cGMP environments.
-
Additional resources
- Viral vector tips
- Regulatory insights
-
Related applications
- Cell therapy
-
Other gene delivery
- AAV vector
- Adenovirus
-
Additional resources
- Viral vector tips
- Regulatory insights
-
Related applications
- Cell therapy
-
Other gene delivery
- AAV vector
- Adenovirus
Lentiviral vector production using single-use bioreactors
A robust and scalable process in HEK293T cells with HyClone™ peak expression medium for growth and transfection.
Primary clarification of lentiviral vectors
In this study, we discuss development of a primary clarification protocol to separate LV from bioreactor-derived suspension cells and debris using a scalable filter train.
Improving lentiviral vector downstream processing workflows
In this article we discuss process development pain points of a modern downstream workflow and ways to address them.
Lentivirus purification
In this study, we share an alternative lentivirus purification process using capture with weak anion exchange and optimization of the Capto™ Core 700 resin polishing step to maximize infectious recovery.
ÄKTA ready™ 450
Single-use chromatography system for small-scale manufacturing.
Capto™ Core 700 resin
Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality.
Stax™ disposable depth filter
Highly versatile disposable depth filter platform.
Xcellerex™ XDR bioreactor
Single-use bioreactor systems providing scalable and robust stirred-tank performance for up to 2000 L in both cGMP and non-cGMP environments.
-
Additional resources
- Viral vector tips
- Regulatory insights
-
Related applications
- Cell therapy
-
Other gene delivery
- AAV vector
- Adenovirus
-
Additional resources
- Viral vector tips
- Regulatory insights
-
Related applications
- Cell therapy
-
Other gene delivery
- AAV vector
- Adenovirus